The Effect of Conditioned Medium of Adipose-Derived Stem Cells on the Expression Level of Cartilage Specific Genes by Chondrocytes

Seyyedeh Mahdiye Hatami; Sina Parsay; Maryam Hassan Famian; Masoumeh Kazemi; Azadeh Montaseri

E-ISSN : 2148-9696

Crescent Journal of
Medical and Biological Sciences

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Jul 2020, Vol 7, Issue 3

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Original Article
The Effect of Conditioned Medium of Adipose-Derived Stem Cells on the Expression Level of Cartilage Specific Genes by Chondrocytes
Seyyedeh Mahdiye Hatami¹, Sina Parsay², Maryam Hassan Famian¹, Masoumeh Kazemi³, Azadeh Montaseri³
¹Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran ²Student’s Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran ³Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
CJMB 2020; 7: 409-413 Viewed : 3296 times Downloaded : 1848 times. Keywords : Stem cells, Adipose tissue, Osteoarthritis, Supernatant
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Abstract

Objectives: Osteoarthritis (OA) is the most common multifactorial disease that deteriorates articular cartilage in the elderly. Recently, mesenchymal stem cells have been introduced as candidates for OA treatment but some researchers showed that implantation of stem cells into the lesion site of patients can lead to some side effects. Accordingly, many scientists switch their field of research to the application of stem cells supernatant. The purpose of this study is to evaluate whether supernatant of adipose-derived stem cells (ASCs) can result in the expression of cartilage-specific genes in chondrocytes. Materials and Methods: Mesenchymal stem cells were isolated from adipose tissue and their supernatant was collected and added to chondrocytes. After 3 days, chondrocytes were prepared for expression of collagen type II, sox9, COMP and aggrecan genes using real-time RT-PCR. Results: Data analysis showed that in the presence of ASCs conditioned medium (CM), the gene expression level of collagen II, sox9, and aggrecan did not change but that of the COMP significantly increased after 3 days. Conclusions: The obtained results may be related to the unknown factors secreted by stem cells and more investigations are necessary for understanding the exact mechanisms underlying these findings.