|The Assessment of Albendazole and Mebendazole Effects on the Excretory / Secretory Proteome of G1 strain of Echinococcusgranulosus Protoscoleces, Using Two-Dimensional Gel Electrophoresis|
|Mohsen Eshraghi1, Seyed Jafar Adnani Sadati2,3, Ali Farahank4, Ali Farahank4, Hakimeh Zali5, Babak Aghili3|
|1Department of Surgery, Sh. Beheshty Hospital, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran
2Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
3Department of Medical Microbiology and Immunology, Faculty of Medicine,Qom University of Medical Sciences, Qom, Iran
4Department of Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran
5School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical sciences,Tehran
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Keywords : Protoscoleces, Albendazole, Mebendazole, Proteome, Two -Dimensional Gel Electrophoresis
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Objectives: The larval stage of Echinococcusgranulosus, in internal organs of human causes cystic hydatidosis. Identification of Excretory/ Secretory (E/S) proteins from E. granulosusprotoscoleces may help to discover new candidates for drug targets, immunodiagnostic and vaccine products. The purpose of this study was assessment of the efficacy of Albendazole and Mebendazole on protein spots of E/S products of hydatid cyst protoscolices which can be helpful for detecting some target proteins for therapeutic purposes.
Materials and Methods:In this experimental study, in order to assess the drug's effects; protoscoleces were divided into three groups. The first and the second groups were treated with Albendazole/Mebendazole respectively and the third group was considered as control. To determine the proteome spots, the E/S proteins were precipitated with TCA/acetone and load on IEF gel, resulting gel differentiated on SDS-PAGE with 20mA constant current. We stained the gels with Coomasie Brilliant Blue. The protein spots resulted from 2-DE gels were analyzed using the Progenesis Same spots Software.
Results: The comparison between the proteome gels of treated groups and control group showed that 35 protein spots are paired, that 11 the protein spots had significant differences in their expression(p<0.05).
Conclusions:Comparison between the levels of expression of protein gel spots indicate increasing expression of some protein spots and suppression of the others. This suppression can be considered as a specific effect of the drugs on the E/S product of protoscoleces of hydatid cyst.
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