Materials and Methods: This study investigates the effects of pretreatment of BS oil with various concentrations (0 (control sample), 2.5, 5, 7.5 and 10%) of β-cyclodextrin dissolved in water: ethanol at ratios of 1:1 or 1:2 (v/v) on thymoquinone, chlorophyll, carotenoids, peroxide value (PV) and acid value (AV), oxidative stability and fatty acids. High-performance liquid chromatography and spectrometry determined thymoquinone content, chlorophyll, and carotenoid amount. PV and AV were measured by titration. Oxidative stability was determined using the Rancimat equipment. Fatty acid composition was also determined by gas chromatography.

Results: The results revealed that β-cyclodextrin, up to 5% dissolved in water: ethanol (1:2, v/v), could significantly reduce the PV (16.9 to 10.2 meq O2/kg oil) and AV (4.2 to 2.7 mg KOH/g oil) and increase the oxidative stability (9.8 to 12.7 h). Moreover, β-cyclodextrin could also reduce the bioactive components (thymoquinone and carotenoids). Still, these reductions were smaller when the oil samples were treated with β-cyclodextrin at concentrations below 7.5%. However, pretreatment of BS oil with β-cyclodextrin did not affect the fatty acid composition of the oil samples.

Conclusions: Overall, the findings suggest that β-cyclodextrin treatment (up to 5%) can be an effective strategy to enhance the stability of black seed oil, expanding its potential applications in the food, pharmaceutical, and cosmetic industries.